Fluorometric assay of malic acid and substituted derivatives.

  title={Fluorometric assay of malic acid and substituted derivatives.},
  author={M. Strassman and L. Ceci and A. Tucci},
  journal={Analytical biochemistry},
  volume={23 3},
Abstract A fluorometric assay is described for the determination of α- and β-substituted malic acids. The procedure involves production of a β-keto acid and subsequent condensation with resorcinol to yield fluorescent derivatives of 7-hydroxycoumarin. The assay was shown to be useful over a wide range of concentrations, and is sensitive enough to be used for less than microgram quantities of α-substituted malic acids. 
5 Citations
Fluorometric assay of enzymatic reactions involving acetyl Coenzyme A in aldol condensations.
The optimal conditions for the formation of these umbelliferones from substituted malic acids and resorcinol have been determined and forms the basis for a rapid and sensitive fluoremetric assay of enzymes catalyzing aldol condensation reactions involving acetyl coenzyme A. Expand
Absolute Configuration of a Metabolite in the m-Fission Pathway of Protocatechuate
An aldolase, which is induced in Pseudomonas testosteroni during growth with p-hydroxybenzoate, preferentially attacks the R form of 4-hydroxy-4-methyl-2-oxoglutarate, a metabolite of protocatechuateExpand
Die α-Isopropylamlat-Synthetase bei Hydrogenomonas H 16
Summary1.α-Isopropylmalate synthetase, the first enzyme of the leucine biosynthetic pathway, has been investigated in crude extracts of cells of Hydrogenomonas H 16 and of auxotrophic mutants derivedExpand


The fluorometric determination of malic acid.
  • J. Hummel
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • 1949
A sensitive and specific chemical method for the estimation of malic acid in biological materials has long been desired and the quantitative precipitation of calcium malate by alcohol as a means of fractionation from biological materials gives it additional specificity. Expand
A study of acetyl-CoA condensation with α-keto acids
Abstract The condensation of acetyl-CoA with α-ketobutyrate and with α-ketovalerate to yield α-ethylmalic acid and α-( n -propyl)malic acid, respectively, has been demonstrated to occur in theExpand
Homocitric acid accumulation by a lysine-requiring yeast mutant.
The excretion of homocitric acid by a lysine-requiring yeast mutant and its conversion into lysin convincingly establish the biosynthesis of lysines in yeast via the previously proposed scheme involving homologues of the citric acid cycle. Expand
Enzymatic formation of alpha-isopropylmalic acid, an intermediate in leucine biosynthesis.
When the distribution of activity was determined in leucine samples isolated from yeast grown on various C’4-labeled acetates and lactates, it was found that both carbons of acetate and the cy and 0carbons of lactate gave rise to highly labeled leucines, indicating that these carbonsof the two amino acids probably originate from a common source. Expand
[Detection and determination of acetoacetic acid in the urine].