Fluorometric Studies of Oxidative Metabolism in Isolated Papillary Muscle of the Rabbit

@article{Chapman1972FluorometricSO,
  title={Fluorometric Studies of Oxidative Metabolism in Isolated Papillary Muscle of the Rabbit},
  author={James Chapman},
  journal={The Journal of General Physiology},
  year={1972},
  volume={59},
  pages={135 - 154}
}
The fluorometric technique for measuring the levels of reduced pyridine nucleotides was used to study oxidative metabolism in isolated rabbit papillary muscle at 23 degrees C. The 100% standard level of tissue fluorescence was defined as that measured for muscles resting in oxygenated 10 mM pyruvate solution. This level increased 15% with anoxia and decreased 45% with stimulation in substrate-free solution. Thus, about one-half of the standard tissue fluorescence was metabolically labile and… CONTINUE READING