Fluorometric Assay to Detect the Germination of Bacillus Anthracis Spores and the Germination Inhibitory Effects of Antibodies

Abstract

A sensitive assay for germination of spores of the anthrax agent was developed using a fluorescence reader. Significant inhibition of germination was detected for spores initially treated with antispore or antitoxin antibodies. Antigens specific to ungerminated spores that stimulate potentially protective antibodies are being identified and their role in… (More)

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