Fluorogenic reactions for biomedical chromatography.


A number of fluorogenic reactions, which have been used for HPLC detection systems by means of pre- and/or postcolumn derivatization, are surveyed with respect to both sensitivity and selectivity for the determination of biomedically important substances. For the derivatization of the substances, two types of fluorogenic reactions, fluorescence-generating and fluorescence-tagging, have been studied. The former are usable in most instances for both pre- and postcolumn derivatization methods, and the latter only for precolumn derivatization methods. HPLC methods utilizing the fluorogenic reactions allow analytes to be detected at picomole-subfemtomole levels. In the fluorescence-generating reactions, several fluorogenic reagents possessing two or more reactive sites in the molecule, which show molecular recognition for a variety of analytes, permit facile and reproducible detection in HPLC because there are fewer interferences from biological matrices.


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@article{Ohkura1994FluorogenicRF, title={Fluorogenic reactions for biomedical chromatography.}, author={Y Ohkura and M Kai and H Nohta}, journal={Journal of chromatography. B, Biomedical applications}, year={1994}, volume={659 1-2}, pages={85-107} }