Fluorogenic DNA ligase and base excision repair enzyme assays using substrates labeled with single fluorophores.

Abstract

Continuing our work on fluorogenic substrates labeled with single fluorophores for nucleic acid modifying enzymes, here we describe the development of such substrates for DNA ligases and some base excision repair enzymes. These substrates are hairpin-type synthetic DNA molecules with a single fluorophore located on a base close to the 3' ends, an… (More)
DOI: 10.1016/j.ab.2015.02.022

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Cite this paper

@article{Nikiforov2015FluorogenicDL, title={Fluorogenic DNA ligase and base excision repair enzyme assays using substrates labeled with single fluorophores.}, author={Theo T Nikiforov and Steven Roman}, journal={Analytical biochemistry}, year={2015}, volume={477}, pages={69-77} }