Fluorimetric determination of ascorbic acid with o-phenylenediamine.

@article{Wu2003FluorimetricDO,
  title={Fluorimetric determination of ascorbic acid with o-phenylenediamine.},
  author={Xia Wu and Yuxia Diao and Changxia Sun and Jinghe Yang and Yuebo Wang and Shuna Sun},
  journal={Talanta},
  year={2003},
  volume={59 1},
  pages={95-9}
}
A simple and sensitive fluorimetric method for the determination of ascorbic acid (AA) is described. The method is based on the condensation reaction between AA and o-phenylenediamine (OPDA) in the absence of the oxidant. The fluorescence intensity is measured at excitation and emission wavelengths of 360 and 430 nm, respectively. Under optimum condition, a linear relationship is obtained between the fluorescence intensity and the concentration of AA in the range of 0.05-40 mug ml(-1). The… CONTINUE READING

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