Fluoride and bovine bone extract influence cell proliferation and phosphatase activities in human bone cell cultures.

@article{Wergedal1988FluorideAB,
  title={Fluoride and bovine bone extract influence cell proliferation and phosphatase activities in human bone cell cultures.},
  author={Jon E. Wergedal and Ken H. Lau and David J. Baylink},
  journal={Clinical orthopaedics and related research},
  year={1988},
  volume={233},
  pages={274-82}
}
The effects of fluoride (20 mumol/L) and bovine bone extract (17 micrograms/ml) were determined on cultures of human bone cells, embryonic chick bone cells, and human skin fibroblasts. The incorporation of [3H]thymidine into DNA was measured 16 hours after the addition of factors. After three to five days treatment, Triton X-100 extracts of the cells were assayed for acid phosphatase activity, in the presence and absence of tartrate, and for alkaline phosphatase activity. Fluoride stimulated… CONTINUE READING