Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells.

Abstract

We investigated the accuracy of fluorescein diacetate uptake as an indicator of the viability of human fetal cerebral cortical cells. Cortical cells from 16-26-week-old normal fetuses were studied. The cortices were dissociated mechanically with normal saline to make a suspension. Fluorescein diacetate uptake and trypan blue exclusion were compared as methods for examining cell viability. Our results show that fluorescein diacetate uptake is a simple and sensitive method for examining human fetal cortical cell viability.

Cite this paper

@article{Zhang1995FluoreseinDU, title={Fluoresein diacetate uptake to determine the viability of human fetal cerebral cortical cells.}, author={F Zhang and P Zhang and Erin Yaoling Wang and Fong-Chin Su and Yuan Qi and X Y Bu and Y Wang}, journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission}, year={1995}, volume={70 4}, pages={185-7} }