Fluorescent-tagged heparan sulfate precursor oligosaccharides to probe the enzymatic action of heparitinase I.

@article{Babu2010FluorescenttaggedHS,
  title={Fluorescent-tagged heparan sulfate precursor oligosaccharides to probe the enzymatic action of heparitinase I.},
  author={Ponnusamy Babu and Balagurunathan Kuberan},
  journal={Analytical biochemistry},
  year={2010},
  volume={396 1},
  pages={124-32}
}
Heparitinase I, a key lyase enzyme essential for structural analysis of heparan sulfate (HS), degrades HS domains that are undersulfated at glucuronyl residues through an elimination mechanism. Earlier studies employed viscosimetric measurements and electrophoresis to deduce the mechanism of action of heparitinase I and two other related lyases, heparitinase II and heparitinase III. However, these findings lack molecular evidence for the intermediates formed and could not distinguish whether… CONTINUE READING