Fluorescent staining of kappa opioid receptors using naltrexamine derivatives and phycoerythrin.

@article{Lawrence1997FluorescentSO,
  title={Fluorescent staining of kappa opioid receptors using naltrexamine derivatives and phycoerythrin.},
  author={D Meinert Lawrence and Ian I. Hutchinson and Ahmad Seyed-Mozaffari and Stephen B Archer and Jean M Bidlack},
  journal={Journal of immunological methods},
  year={1997},
  volume={201 2},
  pages={
          173-81
        }
}
An immunofluorescent technique that is more sensitive than radioligand binding was developed in order to detect opioid receptors expressed on leukocytes. The current study was designed to optimize the method for fluorescently labeling kappa opioid receptors. For these experiments, the opioid antagonist naltrexamine was conjugated to either fluorescein (FITC-NTXamine) or biotin (biotin-NTXamine). One-step, two-step, and three-step protocols were compared to determine which procedure resulted in… CONTINUE READING