Fluorescent staining of acetylcholine receptors in vertebrate skeletal muscle.

@article{Anderson1974FluorescentSO,
  title={Fluorescent staining of acetylcholine receptors in vertebrate skeletal muscle.},
  author={Marilyn J. Anderson and Monroe W. Cohen},
  journal={The Journal of physiology},
  year={1974},
  volume={237 2},
  pages={385-400}
}
1. alpha-Bungarotoxin was labelled with fluorescent dyes and used as a stain for visualizing the distribution of acetylcholine receptors in vertebrate skeletal muscle fibres.2. Dye-toxin conjugates had the same pharmacological properties as native toxin, but their potencies were lower.3. Fluorescent staining was examined in teased muscle fibres. The stain was found to be confined to the neuromuscular junction and associated with the subsynaptic membrane.4. Staining intensity was reduced by… CONTINUE READING
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