Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferase.

Abstract

Fluorescent tagging of proteins has become a critical step in optical analysis of protein function in vitro and in living cells. Here we describe a two-tag system for expression and isolation of a protein of interest from Escherichia coli and subsequent site-specific fluorescent labeling with Sfp phosphopantetheinyl transferase (Sfp synthase). In the… (More)
DOI: 10.1007/978-1-61737-967-3_18

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Cite this paper

@article{Zhang2011FluorescentSL, title={Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferase.}, author={Aihua Zhang and Luo Sun and John A. Buswell and Nancy Considine and Inca Ghosh and Anastasiya Masharina and Christopher Noren and Ming-Qun Xu}, journal={Methods in molecular biology}, year={2011}, volume={705}, pages={295-307} }