Fluorescent sensors for Zn(2+) based on a fluorescein platform: synthesis, properties and intracellular distribution.

@article{Burdette2001FluorescentSF,
  title={Fluorescent sensors for Zn(2+) based on a fluorescein platform: synthesis, properties and intracellular distribution.},
  author={Shawn C. Burdette and Grant Walkup and Bernhard Spingler and Roger Tsien and Stephen J. Lippard},
  journal={Journal of the American Chemical Society},
  year={2001},
  volume={123 32},
  pages={
          7831-41
        }
}
Two new fluorescent sensors for Zn(2+) that utilize fluorescein as a reporting group, Zinpyr-1 and Zinpyr-2, have been synthesized and characterized. Zinpyr-1 is prepared in one step via a Mannich reaction, and Zinpyr-2 is obtained in a multistep synthesis that utilizes 4',5'-fluorescein dicarboxaldehyde as a key intermediate. Both Zinpyr sensors have excitation and emission wavelengths in the visible range ( approximately 500 nm), dissociation constants (K(d1)) for Zn(2+) of <1 nM, quantum… 

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