Fluorescent proteins for live cell imaging: opportunities, limitations, and challenges.

  title={Fluorescent proteins for live cell imaging: opportunities, limitations, and challenges.},
  author={J{\"o}rg Wiedenmann and Franz Oswald and Gerd Ulrich Nienhaus},
  journal={IUBMB life},
  volume={61 11},
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview… CONTINUE READING

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