Fluorescent proteins as proteomic probes.

  title={Fluorescent proteins as proteomic probes.},
  author={Ileana M. Cristea and Rosemary M Williams and Brian T Chait and Michael P. Rout},
  journal={Molecular & cellular proteomics : MCP},
  volume={4 12},
Protein complexes mediate the majority of cellular processes. Knowledge of the localization and composition of such complexes provides key insights into their functions. Although green fluorescent protein (GFP) has been widely applied for in vivo visualization of proteins, it has been relatively little used as a tool for the isolation of protein complexes. Here we describe the use of the standard GFP tag to both visualize proteins in living cells and capture their interactions via a simple… CONTINUE READING

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Closing mitosis : the functions of the Fluorescent Proteins as Proteomic Probes 1940

  • F. Stegmeier, A. Amon
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  • 2004

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  • Annu. Rev. Genet
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