Fluorescent proteins and their applications in imaging living cells and tissues.

@article{Chudakov2010FluorescentPA,
  title={Fluorescent proteins and their applications in imaging living cells and tissues.},
  author={D. Chudakov and M. Matz and S. Lukyanov and K. Lukyanov},
  journal={Physiological reviews},
  year={2010},
  volume={90 3},
  pages={
          1103-63
        }
}
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from diverse marine animals are widely used as universal genetically encoded fluorescent labels. Many laboratories have focused their efforts on identification and development of fluorescent proteins with novel characteristics and enhanced properties, resulting in a powerful toolkit for visualization of structural organization and dynamic processes in living cells and organisms. The diversity of currently… Expand
GREEN FLUORESCENT PROTEIN AND THEIR APPLICATIONS IN ADVANCE RESEARCH
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from diverse marine animals are widely used in biological science as a universal genetically encoded fluorescentExpand
Green Fluorescent Protein (GFP)
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There is still room for further improvement of these important markers for live cell imaging, and special FP variants with low switching fatigue have been introduced in recent years. Expand
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Three strategies for fluorescent labeling of proteins of interest including self-labeling enzyme derivatives, stainable peptide tags and non-canonical amino acid incorporation are summarized, which greatly expand the fluorescent labeling toolbox and provide new opportunities for imaging biological processes. Expand
Chemical biology-based approaches on fluorescent labeling of proteins in live cells.
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Synthetic fluorescent probes are smaller than fluorescent proteins, often have improved photochemical properties, and offer a larger variety of colors, and can be introduced to POIs selectively by numerous approaches that can be largely categorized into chemical recognition- based labeling, and biological recognition-based labeling. Expand
Rapid Directed Molecular Evolution of Fluorescent Proteins in Mammalian Cells
TLDR
Using this method, screening of large gene libraries containing up to 2·107 independent random genes of fluorescent proteins expressed in HEK cells completing one iteration directed evolution in a course of ∼8 days was able to be performed. Expand
Development and application of fluorescent protein-based indicators for live cell imaging
TLDR
The advantages of FP-based indicators extend their application to understanding the functions of particular molecules and cellular events during physiological responses in live animals. Expand
Novel uses of fluorescent proteins.
TLDR
In addition to commonly used GFP-like proteins, unrelated types of FPs on the base of flavin- binding domains, bilirubin-binding domain or biliverdin-binding domains were designed, which opened previously unexplored niches of FP uses such as labeling under anaerobic conditions, deep tissue imaging and even patients' blood analysis. Expand
Live‐cell imaging of cell signaling using genetically encoded fluorescent reporters
TLDR
Recent advances in protein engineering are highlighted that are likely to help expand and improve the design and application of genetically encoded fluorescent reporters as an important platform for advancing the understanding of G protein‐coupled receptor signaling and neuronal activity. Expand
Chromophore photophysics and dynamics in fluorescent proteins of the GFP family.
  • K. Nienhaus, G. Nienhaus
  • Chemistry, Medicine
  • Journal of physics. Condensed matter : an Institute of Physics journal
  • 2016
TLDR
This topical review presents an overview of the key structural and spectroscopic properties of fluorescent proteins, and addresses protein-chromophore interactions that govern ground state optical properties as well as processes occurring in the electronically excited state. Expand
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