Fluorescent properties of monoamine neurons following glyoxylic acid treatment of intact leech ganglia

@article{Lent1982FluorescentPO,
  title={Fluorescent properties of monoamine neurons following glyoxylic acid treatment of intact leech ganglia},
  author={Charles M. Lent},
  journal={Histochemistry},
  year={1982},
  volume={75},
  pages={77-89}
}
The SPG modification (de la Torre and Surgeon 1976) of the glyoxylic acid method for amine condensation is a straightforward procedure which can be used upon intact ganglia from the leech. Intense fluorescence of the neurosomata of identified neurons which contain either indoleamine (serotonin, 5-HT) or catecholamine (CA) is obtained in less than 30 min. The fluorescence of the 5-HT containing neurons is yellow (518–526 nm) and decays more rapidly than the dominant blue emission (478–480 nm) of… CONTINUE READING