Fluorescent probes for live-cell RNA detection.

  title={Fluorescent probes for live-cell RNA detection.},
  author={Gang Bao and Won Jong Rhee and Andrew Tsourkas},
  journal={Annual review of biomedical engineering},
Commonly used techniques for analyzing gene expression, such as polymerase chain reaction (PCR), microarrays, and in situ hybridization, have proven invaluable in understanding RNA processing and regulation. However, these techniques rely on the use of lysed and/or fixed cells and are therefore limited in their ability to provide important spatial-temporal information. This has led to the development of numerous techniques for imaging RNA in living cells, some of which have already provided… CONTINUE READING