Fluorescent probes for imaging endogenous β-actin mRNA in living cells using fluorescent protein-tagged pumilio.

@article{Yoshimura2012FluorescentPF,
  title={Fluorescent probes for imaging endogenous β-actin mRNA in living cells using fluorescent protein-tagged pumilio.},
  author={Hideaki Yoshimura and Asumi Inaguma and Toshimichi Yamada and Takeaki Ozawa},
  journal={ACS chemical biology},
  year={2012},
  volume={7 6},
  pages={999-1005}
}
Subcellular localization and dynamics of mRNAs control various physiological functions in living cells. A novel technique for visualizing endogenous mRNAs in living cells is necessary for investigation of the spatiotemporal movement of mRNAs. A pumilio homology domain of human pumilio 1 (PUM-HD) is a useful RNA binding protein as a tool for mRNA recognition because the domain can be modified to bind a specific 8-base sequence of target mRNA. In this study, we designed PUM-HD to match the… CONTINUE READING

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