Fluorescent oligonucleotide ligation technology for identification of ras oncogene mutations.

@article{Eggerding2000FluorescentOL,
  title={Fluorescent oligonucleotide ligation technology for identification of ras oncogene mutations.},
  author={Faye A. Eggerding},
  journal={Molecular biotechnology},
  year={2000},
  volume={14 3},
  pages={223-33}
}
A mutation detection strategy based on multiplex PCR followed by multiplex allele-specific oligonucleotide probe ligation was developed to detect single nucleotide substitutions in ras oncogenes, a common genetic abnormality in many human cancers. Mutation-specific probes are synthesized for each possible single-base, nonsilent mutation in codons 12, 13, and 61 of H-, K-, and N-ras oncogenes. Mutations are identified by competitive oligonucleotide probe ligation to detect normal and/or mutant… CONTINUE READING