Fluorescent molecular rotors: a new class of probes for tubulin structure and assembly.

@article{Kung1989FluorescentMR,
  title={Fluorescent molecular rotors: a new class of probes for tubulin structure and assembly.},
  author={Chin-Sheng Kung and Juta K. Reed},
  journal={Biochemistry},
  year={1989},
  volume={28 16},
  pages={
          6678-86
        }
}
9-(Dicyanovinyl)julolidine (DCVJ) is a fluorescent dye whose intramolecular rotational relaxation is solvent dependent. Since its quantum yield increases with decreasing free volume, this molecule has been very useful in monitoring synthetic polymer reactions and measuring local microviscosity changes in phospholipid bilayers [Loutfy, R. O. (1986) Pure Appl. Chem. 58, 1239-1248; Kung, C. E., & Reed, J. K. (1986) Biochemistry 25, 6114-6121]. We have used DCVJ to follow the polymerization of… CONTINUE READING
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