Fluorescent labeling of a carboxyl group of sialic acid for MALDI-MS analysis of sialyloligosaccharides and ganglioside.

@article{Endo2009FluorescentLO,
  title={Fluorescent labeling of a carboxyl group of sialic acid for MALDI-MS analysis of sialyloligosaccharides and ganglioside.},
  author={Shin-ichi Endo and Minoru Morita and Masaki Ueno and Tadakazu Maeda and Takashi Terabayashi},
  journal={Biochemical and biophysical research communications},
  year={2009},
  volume={378 4},
  pages={890-4}
}
We developed a modified method enabling stable MALDI-MS analysis and fluorescent detection of sialyl-compounds. The modification involved the amidation of sialic acid (Neu5Ac) at the position of the carboxyl group using the fluorescent reagent, 2-(2-pyridilamino)ethylamine (PAEA). In this study the following sialyl-compounds were amidated, 3'-sialyllactose (3'-SL), 6'-sialyllactose (6'-SL), and ganglioside GM3. Yields of PAEA-3'-SL, PAEA-6'-SL, and PAEA-GM3 were 45%, 60%, and 30%, respectively… CONTINUE READING

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