Fluorescent in situ visualization of sterols in Arabidopsis roots

  title={Fluorescent in situ visualization of sterols in Arabidopsis roots},
  author={Yohann Boutt{\'e} and Shuzhen Men and Markus Grebe},
  journal={Nature Protocols},
Sterols are eukaryotic membrane components with crucial roles in diverse cellular processes. Elucidation of sterol function relies on development of tools for in situ sterol visualization. Here we describe protocols for in situ sterol localization in Arabidopsis thaliana root cells, using filipin as a specific probe for detection of fluorescent filipin-sterol complexes. Currently, filipin is the only established tool for sterol visualization in plants. Filipin labeling can be performed on… CONTINUE READING
15 Citations
44 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 15 extracted citations


Publications referenced by this paper.
Showing 1-10 of 44 references

Similar Papers

Loading similar papers…