Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues

@article{Lee2015FluorescentIS,
  title={Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues},
  author={Je Hyuk Lee and Evan R. Daugharthy and Jonathan Scheiman and Reza Kalhor and Thomas C. Ferrante and Richard W. Terry and Brian Turczyk and Joyce L Yang and Ho Suk Lee and John Aach and Kun Qiu Zhang and George M Church},
  journal={Nature Protocols},
  year={2015},
  volume={10},
  pages={442-458}
}
RNA-sequencing (RNA-seq) measures the quantitative change in gene expression over the whole transcriptome, but it lacks spatial context. In contrast, in situ hybridization provides the location of gene expression, but only for a small number of genes. Here we detail a protocol for genome-wide profiling of gene expression in situ in fixed cells and tissues… CONTINUE READING