Fluorescent in situ hybridization on tissue microarrays: challenges and solutions

@article{Brown2006FluorescentIS,
  title={Fluorescent in situ hybridization on tissue microarrays: challenges and solutions},
  author={Lindsay A. Brown and David G. Huntsman},
  journal={Journal of Molecular Histology},
  year={2006},
  volume={38},
  pages={151-157}
}
Tissue microarray (TMA) technology has provided a high throughput means of evaluating potential biomarkers and therapeutic targets in archival pathological specimens. TMAs facilitate the rapid assessment of molecular alterations in hundreds of different tumours on a single slide. Sections from TMAs can be used for any in situ tissue analysis, including fluorescent in situ hybridization (FISH). FISH is a molecular technique that detects numerical and structural abnormalities in both metaphase… Expand
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TLDR
FISH analysis on TMA has been successfully used not only to uncover genetic alterations in various malignancies but to also rapidly establish the clinical significance of such changes. Expand
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A comprehensive morphological approach to pathology archives that consists of fluorescence in situ hybridization with bacterial artificial chromosome probes and screening with tissue microarrays to detect structural changes in chromosomes in specimens of human solid tumors is reviewed. Expand
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TLDR
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TLDR
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TLDR
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