Fluorescent detection of alpha-aminoadipic and gamma-glutamic semialdehydes in oxidized proteins.

@article{Akagawa2009FluorescentDO,
  title={Fluorescent detection of alpha-aminoadipic and gamma-glutamic semialdehydes in oxidized proteins.},
  author={Mitsugu Akagawa and Kyozo Suyama and Koji Uchida},
  journal={Free radical biology & medicine},
  year={2009},
  volume={46 6},
  pages={701-6}
}
The oxidative modification of proteins is believed to play a critical role in the etiology and/or progression of several diseases. alpha-Aminoadipic semialdehyde (AAS) and gamma-glutamic semialdehyde (GGS) residues represent major oxidized amino acids generated in oxidized proteins. This paper describes a novel procedure for the specific and sensitive determination of AAS and GGS after their reductive amination with sodium cyanoborohydride and p-aminobenzoic acid, a fluorescence reagent, to… CONTINUE READING