Fluorescent biosensors to investigate helicase activity.

Abstract

ATP-driven translocation of helicases along DNA can be assayed in several ways. Reagentless biosensors, based on fluorophore-protein adducts, provide convenient ways for real-time assays of both the separation of dsDNA and the hydrolysis of ATP. Single-stranded DNA can be assayed using a modified single-stranded DNA-binding protein (SSB), and phosphate… (More)
DOI: 10.1007/978-1-60327-355-8_2

Topics

4 Figures and Tables

Slides referencing similar topics