Fluorescence visualization of cellulose and pectin in the primary plant cell wall

@article{Bidhendi2020FluorescenceVO,
  title={Fluorescence visualization of cellulose and pectin in the primary plant cell wall},
  author={Amir J. Bidhendi and Youssef Chebli and Anja Geitmann},
  journal={Journal of Microscopy},
  year={2020},
  volume={278}
}
Plant cell walls constitute the extracellular matrix surrounding plant cells and are composed mainly of polysaccharides. The chemical makeup of the primary plant cell wall, and specifically, the abundance, localization and arrangement of the constituting polysaccharides are intimately linked with growth, morphogenesis and differentiation in plant cells. Visualization of the cell wall components is, therefore, a crucial tool in plant cell developmental studies. In this technical update, we… 
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References

SHOWING 1-10 OF 130 REFERENCES
Small Molecule Probes for Plant Cell Wall Polysaccharide Imaging
TLDR
This review of the currently available molecular toolbox of probes for cell wall polysaccharide imaging in plants is summarized, with particular emphasis on recent advances in small molecule-based fluorescent probes.
Advances in Imaging Plant Cell Walls.
Molecular probes for the plant cell surface
The precise deposition of the plant cell wall, in terms of orientation and timing, dictates the anatomy of a plant and the form of all plant structures. The varied cell fates within a developing
Fluorescent Probes for Exploring Plant Cell Wall Deconstruction: A Review
TLDR
Fluorescent probes bearing various fluorophores have been devised and used successfully to reveal the changes in structural motifs during plant biomass deconstruction, and the molecular interactions between enzymes and plant cell wall polymers.
Understanding plant cell morphogenesis requires real-time monitoring of cell wall polymers.
Illuminating the wall
TLDR
The use of click chemistry to investigate plant cell wall architecture should help bridge the gap between biochemical characterization of isolated cell wall components and an understanding of how those components interact in intact cell walls.
Relating the mechanics of the primary plant cell wall to morphogenesis.
TLDR
The major cell wall polysaccharides and their implication for plant cell wall mechanics that need to be considered in order to study the growth behavior of the primary plantcell wall are reviewed.
Monoclonal antibodies, carbohydrate-binding modules, and the detection of polysaccharides in plant cell walls.
TLDR
This work provides an account of methods that can be used to detect cell wall polysaccharide structures (epitopes) in plant materials and also describes treatments that can provide information on the masking of sets ofpolysaccharides that may prevent detection.
Mechanical modeling and structural analysis of the primary plant cell wall.
  • A. Geitmann
  • Biology, Engineering
    Current opinion in plant biology
  • 2010
Restricted access of proteins to mannan polysaccharides in intact plant cell walls.
TLDR
It is shown that molecular recognition of mannan polysaccharides present in intact cell walls is severely restricted and that the masking of primary cell wall mannan by pectin is a potential mechanism for controlling cell wall micro-environments.
...
...