Fluorescence studies of phosphatidylcholine micelle mixing: relevance to phospholipase kinetics.

@article{Soltys1994FluorescenceSO,
  title={Fluorescence studies of phosphatidylcholine micelle mixing: relevance to phospholipase kinetics.},
  author={C E Soltys and Mary Fedarko Roberts},
  journal={Biochemistry},
  year={1994},
  volume={33 38},
  pages={
          11608-17
        }
}
Two fluorescent micellar phospholipid probes (1-hexanoyl-2-(1-pyrenebutyroyl)phosphatidylcholine and 1-octanoyl-2-(1-pyrenebutyroyl)phosphatidylcholine) have been synthesized, characterized, and used to monitor the dynamics of lipid/amphiphile exchange in a variety of detergents and phospholipid micelles using both steady-state and stopped-flow fluorescence techniques. The ratio of the pyrene monomer to excimer band is a good indicator of the extent of lipid mixing at equilibrium. Following the… CONTINUE READING
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