Fluorescence studies of a single tyrosine in a type II DNA binding protein.

@article{Hrd1989FluorescenceSO,
  title={Fluorescence studies of a single tyrosine in a type II DNA binding protein.},
  author={Torleif H{\"a}rd and Victor Hsu and Michael H. Sayre and E. Peter Geiduschek and Krzysztof Appelt and David R. Kearns},
  journal={Biochemistry},
  year={1989},
  volume={28 1},
  pages={396-406}
}
We studied the fluorescence properties of a single tyrosine (Tyr94) located in the C-terminal tail of transcription factor 1 (TF1), a type II procaryotic DNA binding protein encoded by the Bacillus subtilis phage SPO1. The time-resolved fluorescence intensity of Tyr94 in free TF1 dimers decays as a single exponential, and this is consistent with a twofold… CONTINUE READING