Fluorescence spectroscopy of single biomolecules.

  title={Fluorescence spectroscopy of single biomolecules.},
  author={Shimon Weiss},
  volume={283 5408},
  • S. Weiss
  • Published 12 March 1999
  • Chemistry, Physics, Biology
  • Science
Recent advances in single-molecule detection and single-molecule spectroscopy at room temperature by laser-induced fluorescence offer new tools for the study of individual macromolecules under physiological conditions. These tools relay conformational states, conformational dynamics, and activity of single biological molecules to physical observables, unmasked by ensemble averaging. Distributions and time trajectories of these observables can therefore be measured during a reaction without the… 

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Fluorescence from Diffusing Single Molecules Illuminates Biomolecular Structure and Dynamics

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Dual-molecule spectroscopy: molecular rulers for the study of biological macromolecules

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Efficient collection and detection of fluorescence coupled with careful minimization of background from impurities and Raman scattering now enable routine optical microscopy and study of single

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Single-Molecule Fluorescence Analysis in Solution

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Fluorescence lifetimes of single Rhodamine 6G molecules on silica surfaces were measured with pulsed laser excitation, time-correlated single photon counting, and near-field scanning optical