Fluorescence spectroscopy of single biomolecules.

@article{Weiss1999FluorescenceSO,
  title={Fluorescence spectroscopy of single biomolecules.},
  author={Shimon Weiss},
  journal={Science},
  year={1999},
  volume={283 5408},
  pages={
          1676-83
        }
}
  • S. Weiss
  • Published 12 March 1999
  • Chemistry, Physics, Biology
  • Science
Recent advances in single-molecule detection and single-molecule spectroscopy at room temperature by laser-induced fluorescence offer new tools for the study of individual macromolecules under physiological conditions. These tools relay conformational states, conformational dynamics, and activity of single biological molecules to physical observables, unmasked by ensemble averaging. Distributions and time trajectories of these observables can therefore be measured during a reaction without the… 

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References

SHOWING 1-10 OF 89 REFERENCES

Optical studies of single molecules at room temperature.

Recent developments in optical studies of single molecules at room temperature are reviewed, with an emphasis on the underlying principles and the potential of single-molecule experiments. Examples

Monitoring conformational dynamics of a single molecule by selective fluorescence spectroscopy.

The consistent results strongly support the hypothesized three-state model of the conformational dynamics of the TMR-DNA duplex with a polar, a nonpolar, and a quenching environment of TMR.

Single-molecule biomechanics with optical methods.

Experiments with rotary motors, linear motors, RNA polymerase, and titin are described, highlighting important advances in the understanding of how motor proteins work.

Dual-molecule spectroscopy: molecular rulers for the study of biological macromolecules

Single-molecule detection and spectroscopy by laser induced fluorescence offer many applications to life sciences. We review recent techniques and experiments based on the detection and spectroscopy

Probing Single Molecule Dynamics

A movie of the time-resolved emission demonstrates the feasibility of fluorescence lifetime imaging with single molecule sensitivity, picosecond temporal resolution, and a spatial resolving power beyond the diffraction limit.

Illuminating single molecules in condensed matter.

Efficient collection and detection of fluorescence coupled with careful minimization of background from impurities and Raman scattering now enable routine optical microscopy and study of single

Direct Measurement of Single-Molecule Diffusion and Photodecomposition in Free Solution

Continuous monitoring of submillisecond free-solution dynamics of individual rhodamines-6G molecules and 30-base single-stranded DNA tagged with rhodamine was achieved, and stochastic behavior was found for individual molecules of each type, and smaller diffusion coefficients were observed.

Single-Molecule Fluorescence Analysis in Solution

Over the past five years, several groups have developed the capability to detect and identify single fluorescent molecules in solution as the molecules flow through a focused laser beam. The history

Alterations of Single Molecule Fluorescence Lifetimes in Near-Field Optical Microscopy

Fluorescence lifetimes of single Rhodamine 6G molecules on silica surfaces were measured with pulsed laser excitation, time-correlated single photon counting, and near-field scanning optical
...