Fluorescence resonance energy transfer studies of U-shaped DNA molecules.

@article{Lorenz2002FluorescenceRE,
  title={Fluorescence resonance energy transfer studies of U-shaped DNA molecules.},
  author={Mike Lorenz and Alexander Hillisch and S. Diekmann},
  journal={Journal of biotechnology},
  year={2002},
  volume={82 3},
  pages={197-209}
}
Fluorescence resonance energy transfer studies allow to determine global shape properties of nucleic acids and nucleoprotein complexes. In many DNA-protein complexes, the DNA is more or less bent and the degree of bending can be obtained by FRET. For example, the DNA in complex with the integration host factor (IHF) is kinked by approximately 160 degrees building a U-shaped structure. The two DNA helix ends come close to one another in space in a distance range easily measurable by FRET. The… CONTINUE READING