Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in ultradilute hydroxyethylcellulose

  title={Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in ultradilute hydroxyethylcellulose},
  author={J. Schwinefus and S. Wang and R. Hammond and M. Morris},
Fluoresence resonance energy transfer (FRET) microscopy is used to observe structures of DNA aggregates formed during electrophoresis in ultradilute hydroxyethylcellulose (HEC). FRET gives evidence of aggregates with a tightly packed core and random entanglement of DNA in the outer portions. HEC concentrations also appear to play a role in aggregate formation. DNA aggregates take longer to assemble in higher HEC concentration solutions and assume a less compact form than those formed at lower… Expand
6 Citations
Electrohydrodynamically induced aggregation during constant and pulsed field capillary electrophoresis of DNA.
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9 Pulsed-Field Capillary Electrophoresis of


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DNA conformational dynamics in polymer solutions above and below the entanglement limit.
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Dynamics of DNA during pulsed field electrophoresis in entangled and dilute polymer solutions.
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Capillary electrophoresis of DNA in uncross-linked polymer solutions.
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A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.
  • A. Glazer, K. Peck, R. Mathies
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1990
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