Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser.

@article{Karpova2003FluorescenceRE,
  title={Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using confocal microscopy and a single laser.},
  author={Tatiana S. Karpova and Christopher T. Baumann and Longwei He and Xundong Wu and A Grammer and P E Lipsky and Gordon L. Hager and James G. McNally},
  journal={Journal of microscopy},
  year={2003},
  volume={209 Pt 1},
  pages={56-70}
}
One manifestation of fluorescence resonance energy transfer (FRET) is an increase in donor fluorescence after photobleaching the acceptor. Published acceptor-photobleaching methods for FRET have mainly used wide-field microscopy. A laser scanning confocal microscope enables faster and targeted bleaching within the field of view, thereby improving speed and accuracy. Here we demonstrate the approach with CFP and YFP, the most versatile fluorescent markers now available for FRET. CFP/YFP FRET… CONTINUE READING
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