Fluorescence resonance energy transfer analysis of protein-protein interactions in single living cells by multifocal multiphoton microscopy.

@article{Majoul2002FluorescenceRE,
  title={Fluorescence resonance energy transfer analysis of protein-protein interactions in single living cells by multifocal multiphoton microscopy.},
  author={Irina V. Majoul and Martin Straub and Rainer Duden and Stefan W Hell and H D S{\"o}ling},
  journal={Journal of biotechnology},
  year={2002},
  volume={82 3},
  pages={267-77}
}
Fluorescence resonance energy transfer (FRET) resolved by multifocal multiphoton microscopy (MMM) was successfully used to measure transport phenomena in living cells. We expressed different pairs of CFP-/YFP-fusion proteins involved in retrograde Golgi-to-ER transport to analyze sorting of the occupied KDEL-receptor into retrograde transport vesicles triggered by application of the external cholera toxin mutant CTXK63. FRET observed as a sensitized emission of the acceptor was confirmed by… CONTINUE READING

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