Fluorescence recovery after photobleaching studies of lipid rafts.

Abstract

Fluorescence recovery after photobleaching (FRAP) is a microscopy-based technique that can be used to ask how lipid rafts impact protein and lipid diffusion in cells. This chapter, describes how to perform FRAP measurements of putative raft and nonraft proteins and lipids using a confocal microscope. Methods have been outlined for (1) transfecting cells… (More)
DOI: 10.1007/978-1-59745-513-8_13

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Cite this paper

@article{Kenworthy2007FluorescenceRA, title={Fluorescence recovery after photobleaching studies of lipid rafts.}, author={Anne K. Kenworthy}, journal={Methods in molecular biology}, year={2007}, volume={398}, pages={179-92} }