Fluorescence recovery after photobleaching reveals the biochemistry of nucleocytoplasmic exchange.

@article{Bizzarri2012FluorescenceRA,
  title={Fluorescence recovery after photobleaching reveals the biochemistry of nucleocytoplasmic exchange.},
  author={Ranieri Bizzarri and Francesco Cardarelli and Michela Serresi and Fabio Beltram},
  journal={Analytical and bioanalytical chemistry},
  year={2012},
  volume={403 8},
  pages={2339-51}
}
Fluorescence recovery after photobleaching (FRAP) can help unveil subtle dynamical and biochemical properties of intracellular components. A peculiar aspect of this method is that it is based on the change of optical properties only, whereas dynamics and biochemistry of the molecules of interest are not perturbed. This makes FRAP particularly suitable for the study of protein translocation, e.g., between nucleus and cytoplasm. Here we present a comprehensive theoretical treatment of FRAP… CONTINUE READING