Fluorescence properties of pteridine nucleoside analogs as monomers and incorporated into oligonucleotides.

@article{Hawkins1997FluorescencePO,
  title={Fluorescence properties of pteridine nucleoside analogs as monomers and incorporated into oligonucleotides.},
  author={Mary Ellen Hawkins and W. Pfleiderer and Frank M Balis and D Porter and Jay R. Knutson},
  journal={Analytical biochemistry},
  year={1997},
  volume={244 1},
  pages={86-95}
}
Eighteen fluorescent pteridine-based nucleoside analogs have been prepared that are suitable for synthesis as phosphormidites and site-specific incorporation into oligonucleotides. Their quantum yields ranged from < or = 0.03 to 0.88. The maximum excitation and emission wavelenghts of seven selected probes with quantum yields > 0.15 ranged from 334 to 358 and 400 to 444 nm, respectively. Fluorescence decay curves of the seven probes were biexponential, and the mean intensity-weighted lifetimes… CONTINUE READING
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