Fluorescence polarization assay for the diagnosis of bovine brucellosis: adaptation to field use.

Abstract

A fluorescence polarization assay (FPA) was used to test whole blood samples prepared by mixing blood cells from cattle without exposure to Brucella abortus (B. abortus) with sera from animals with confirmed (bacteriologically) infection. A cut-off value between negative and positive values was initially established to be 87.2mP. This value was changed to… (More)

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@article{Nielsen2001FluorescencePA, title={Fluorescence polarization assay for the diagnosis of bovine brucellosis: adaptation to field use.}, author={Klaus Nielsen and Dariusz Gall and Powell Smith and William A. Kelly and J Yeo and Kevin Kenny and Tom Heneghan and Stephen McNamara and Penelope Maher and Johanna O'Connor and Brid Walsh and Jo A Carroll and Ximena Rojas and Fredy Rojas and Blanca Stella Giraldo Perez and O Wulff and L Buffoni and Eduardo Salustio and R J Gregoret and Luis Ernesto Samartino and Antonio Dajer and E Luna-Mart{\'i}nez}, journal={Veterinary microbiology}, year={2001}, volume={80 2}, pages={163-70} }