Fluorescence polarization assay for the detection of antibodies to Mycobacterium bovis in bovine sera.

@article{Jolley2007FluorescencePA,
  title={Fluorescence polarization assay for the detection of antibodies to Mycobacterium bovis in bovine sera.},
  author={Michael E. Jolley and Mohammad Sarwar Nasir and Om P. Surujballi and Anna Romanowska and Tomas B Renteria and Alfonso De la Mora and Ailam Lim and Steven Bolin and Anita L Michel and Miladin Kostovic and Edward C Corrigan},
  journal={Veterinary microbiology},
  year={2007},
  volume={120 1-2},
  pages={113-21}
}
The performance of a fluorescence polarization assay (FPA) that detects antibodies to Mycobacterium bovis in bovine sera is described. The FPA reported here is a direct binding primary screening assay using a small polypeptide derived from the M. bovis MPB70 protein. A secondary inhibition assay confirms suspect or presumed positive samples. Specificity studies involved five different veterinary laboratories testing 4461 presumed negative bovine samples. FPA specificity was 99.9%. The FPA was… CONTINUE READING

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