Fluorescence nanoscopy by ground-state depletion and single-molecule return

@article{Flling2008FluorescenceNB,
  title={Fluorescence nanoscopy by ground-state depletion and single-molecule return},
  author={Jonas F{\"o}lling and Mariano Luis Bossi and Hannes Bock and Rebecca Medda and Christian Andreas Wurm and Birka Hein and Stefan Jakobs and Christian Eggeling and Stefan W Hell},
  journal={Nature Methods},
  year={2008},
  volume={5},
  pages={943-945}
}
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein–labeled (living) samples, proving a simple yet powerful super-resolution approach. 
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