Fluorescence microscopy today

  title={Fluorescence microscopy today},
  author={Rafael Yuste},
  journal={Nature Methods},
  • R. Yuste
  • Published 1 December 2005
  • Biology, Medicine
  • Nature Methods
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues. The impact of these and other new imaging methods in biophysics, neuroscience, and developmental and cell biology has been remarkable. Further advances in fluorophore design, molecular biological tools and nonlinear… 
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Fluorescence microscopy in three dimensions.
The green fluorescent protein.
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In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in
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Two-photon laser scanning fluorescence microscopy.
The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
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