Fluorescence microscopy today

  title={Fluorescence microscopy today},
  author={Rafael Yuste},
  journal={Nature Methods},
  • R. Yuste
  • Published 1 December 2005
  • Biology, Chemistry
  • Nature Methods
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues. The impact of these and other new imaging methods in biophysics, neuroscience, and developmental and cell biology has been remarkable. Further advances in fluorophore design, molecular biological tools and nonlinear… 

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