Fluorescence microscopy today

@article{Yuste2005FluorescenceMT,
  title={Fluorescence microscopy today},
  author={Rafael Yuste},
  journal={Nature Methods},
  year={2005},
  volume={2},
  pages={902-904}
}
  • R. Yuste
  • Published 1 December 2005
  • Biology, Medicine
  • Nature Methods
Fluorescence microscopy has undergone a renaissance in the last decade. The introduction of green fluorescent protein (GFP) and two-photon microscopy has allowed systematic imaging studies of protein localization in living cells and of the structure and function of living tissues. The impact of these and other new imaging methods in biophysics, neuroscience, and developmental and cell biology has been remarkable. Further advances in fluorophore design, molecular biological tools and nonlinear… Expand
Fluorescence lifetime imaging microscopy in life sciences
Fluorescence lifetime imaging microscopy (FLIM) and fluorescence anisotropy imaging microscopy (FAIM) are versatile tools for the investigation of the molecular environment of fluorophores in livingExpand
Accuracy and precision in quantitative fluorescence microscopy
  • J. Waters
  • Medicine, Biology
  • The Journal of cell biology
  • 2009
TLDR
The parameters of digital image acquisition that affect the accuracy and precision of quantitative fluorescence microscopy measurements are focused on. Expand
Advanced Methods in Fluorescence Microscopy
TLDR
The gamut extends from established confocal laser scanning through multiphoton and TIRF to the emerging technologies of super-resolution microscopy that breech the Abbé limit of resolution. Expand
4.09 – Advanced Fluorescence Microscopy
This chapter presents an overview of the physical principles of optical microscopy with emphasis on fluorescence microscopy. After introducing the basic concepts of light, refraction, and imaging, itExpand
Live-Cell Super-resolution Fluorescence Microscopy
TLDR
This review describes some examples of live nanoscopy-based discoveries and focuses on the development of methods and specific fluorescent labeling aimed to decrease the damaging effects of light illumination on live samples. Expand
AI-powered transmitted light microscopy for functional analysis of live cells
TLDR
Artificial-intelligence-powered transmitted light microscopy (AIM) is introduced for subcellular structure identification and labeling-free functional analysis of live cells in their native form without labeling. Expand
Localization and mobility of bacterial proteins by confocal microscopy and fluorescence recovery after photobleaching.
This chapter describes the use of laser-scanning confocal fluorescence microscopy for determining the localization of fluorescently tagged proteins within bacterial cells, discussing the problemsExpand
Fluorescence imaging with tailored light
TLDR
Current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection are discussed, providing a clear overview of their working principles and capabilities. Expand
Resolving Biology Beyond the Diffraction Limit with Single-Molecule Localization Microscopy
TLDR
A short overview of single-molecule localization microscopy is provided and some of its prospects for the future are discussed. Expand
Fluorescence microscopy - avoiding the pitfalls
TLDR
Fluorescence microscopes range from relatively straight-forward wide-field microscopes to highly specialised spectral-imaging confocal, and the development of novel fluorescent probes has put fluorescence microscopy at the center of life science research. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 34 REFERENCES
Optical sectioning microscopy
TLDR
The core concepts of confocal microscopes and important variables that adversely affect confocal images are described and computational optical sectioning techniques that can perform Optical sectioning without a confocal microscope are discussed. Expand
Fluorescence microscopy in three dimensions.
TLDR
This chapter has discussed the nature of image formation in three dimensions and dealt with several means to remove contaminating out-of-focus information and developed a method for extremely rapidly and accurately producing an in-focus, high-resolution "synthetic projection" image from a thick specimen. Expand
The green fluorescent protein.
  • R. Tsien
  • Biology, Medicine
  • Annual review of biochemistry
  • 1998
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins inExpand
Second harmonic imaging microscopy.
TLDR
The chapter provides information on the second harmonic imaging microscopy, a nonlinear optical process that can take place in a microscope that uses illumination from ultrafast (near-infrared) laser light. Expand
Two-photon laser scanning fluorescence microscopy.
TLDR
The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation. Expand
Deep tissue two-photon microscopy
TLDR
Fundamental concepts of nonlinear microscopy are reviewed and conditions relevant for achieving large imaging depths in intact tissue are discussed. Expand
Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.
The diffraction barrier responsible for a finite focal spot size and limited resolution in far-field fluorescence microscopy has been fundamentally broken. This is accomplished by quenching excitedExpand
Scanningless depth-resolved microscopy.
TLDR
By introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning, based on temporal focusing of the illumination pulse. Expand
Second Harmonic Imaging Microscopy
Second Harmonic Generation (SHG) has been developed in our laboratories as a highresolution non-linear optical imaging microscopy (“SHIM”) for cellular membranes and intact tissues. SHG is aExpand
Properties of a 4Pi confocal fluorescence microscope
In a 4Pi confocal fluorescence microscope two opposing microscope objective lenses were used to illuminate a fluorescent object from both sides and to collect the fluorescence emissions on bothExpand
...
1
2
3
4
...