Fluorescence light microscopy of pulmonary surfactant at the air-water interface of an air bubble of adjustable size.

@article{Knebel2002FluorescenceLM,
  title={Fluorescence light microscopy of pulmonary surfactant at the air-water interface of an air bubble of adjustable size.},
  author={Detlef Knebel and Manfred Sieber and Rudolf Reichelt and H J Galla and Matthias A Amrein},
  journal={Biophysical journal},
  year={2002},
  volume={83 1},
  pages={547-55}
}
The structural dynamics of pulmonary surfactant was studied by epifluorescence light microscopy at the air-water interface of a bubble as a model close to nature for an alveolus. Small unilamellar vesicles of dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylglycerol, a small amount of a fluorescent dipalmitoylphosphatidylcholine-analog, and surfactant-associated protein C were injected into the buffer solution. They aggregated to large clusters in the presence of Ca(2+) and adsorbed from… CONTINUE READING