Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.

  title={Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy.},
  author={N{\"o}el Boens and Wenwu Qin and Nikola Basari{\'c} and Johan Hofkens and Marcel Ameloot and Jacques Pouget and Jean-Pierre Lefevre and B. Valeur and Enrico Gratton and Martin Vandeven and Norberto Anibal Pires da Silva and Yves Engelborghs and Katrien Willaert and Alain Sillen and Garry Rumbles and D. Phillips and Antonie J. W. G. Visser and Arie van Hoek and Joseph R. Lakowicz and Henryk Malak and Ignacy Gryczyński and Arthur G. Szabo and Don T Krajcarski and Naoto Tamai and Atsushi Miura},
  journal={Analytical chemistry},
  volume={79 5},
A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 degrees C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all commercially available, are photostable under the conditions of the measurements, and are soluble in… CONTINUE READING


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