Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.

@article{Ge2012FluorescenceLI,
  title={Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission.},
  author={Jianhong Ge and C Kuang and Shin-Shian Lee and F. Kao},
  journal={Optics express},
  year={2012},
  volume={20 27},
  pages={28216-21}
}
We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily… CONTINUE READING