Fluorescence lifetime imaging reveals that the environment of the ATP binding site of myosin in muscle senses force.

@article{IbanezGarcia2010FluorescenceLI,
  title={Fluorescence lifetime imaging reveals that the environment of the ATP binding site of myosin in muscle senses force.},
  author={Delisa Ibanez-Garcia and Jose Requejo-Isidro and Martin R Webb and Timothy G. West and Paul R. French and Michael A Ferenczi},
  journal={Biophysical journal},
  year={2010},
  volume={99 7},
  pages={2163-9}
}
Fluorescence lifetime imaging microscopy is used to demonstrate that different loads applied to a muscle fiber change the microenvironment of the nucleotide binding pocket of myosin. Permeabilized skeletal muscle fibers in rigor were labeled with a fluorescent ATP analog, 3'-DEAC-propylenediamine (pda)-ATP (3'-O-{N-[3-(7-diethylaminocoumarin-3-carboxamido)propyl]carbamoyl}ATP), which was hydrolyzed to the diphosphate. Cycles of small-amplitude stretches and releases (<1% of muscle segment… CONTINUE READING

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