Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scale

@article{Gadella1993FluorescenceLI,
  title={Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scale},
  author={T. Gadella and T. Jovin and R. Clegg},
  journal={Biophysical Chemistry},
  year={1993},
  volume={48},
  pages={221-239}
}
A frequency domain fluorescence lifetime imaging microscope (FLIM) has been developed. A continuous wave laser excitation source of an epi-illumination fluorescence microscope is modulated at a high frequency fA. The lifetime of the modulated fluorescence emission is determined from the phase delay and modulation depth of the fluorescence signal relative to that of the excitation light. Phase detection is accomplished simultaneously at every location in the image by modulating the high voltage… Expand
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