Fluorescence lifetime imaging microscopy (FLIM).

  title={Fluorescence lifetime imaging microscopy (FLIM).},
  author={Erik B van Munster and Theodorus W. J. Gadella},
  journal={Advances in biochemical engineering/biotechnology},
Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems have been implemented both in the frequency domain, using sinusoidally intensity-modulated excitation light and modulated detectors, and in the time domain, using pulsed excitation sources and time-correlated or time-gated detection. In this review we describe the different modes in which both frequency-domain and time… CONTINUE READING
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