Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein.

@article{Pickup2013FluorescenceIA,
  title={Fluorescence intensity- and lifetime-based glucose sensing using glucose/galactose-binding protein.},
  author={John C Pickup and Faaizah Khan and Zheng-Liang Zhi and Jonathan Coulter and David J. S. Birch},
  journal={Journal of diabetes science and technology},
  year={2013},
  volume={7 1},
  pages={62-71}
}
We review progress in our laboratories toward developing in vivo glucose sensors for diabetes that are based on fluorescence labeling of glucose/galactose-binding protein. Measurement strategies have included both monitoring glucose-induced changes in fluorescence resonance energy transfer and labeling with the environmentally sensitive fluorophore, badan. Measuring fluorescence lifetime rather than intensity has particular potential advantages for in vivo sensing. A prototype fiber-optic-based… CONTINUE READING