Fluorescence in situ hybridization using an old world monkey Y chromosome specific probe combined with immunofluorescence staining on rhesus monkey tissues.

@article{Xia2007FluorescenceIS,
  title={Fluorescence in situ hybridization using an old world monkey Y chromosome specific probe combined with immunofluorescence staining on rhesus monkey tissues.},
  author={Xiujin Xia and Terri Rasmussen and Xavier Alvarez and T. Taguchi and Marilyn Li and Vincent F La Russa},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2007},
  volume={55 11},
  pages={1115-21}
}
To date, there is no commercially available Y chromosome probe that can be used for fluorescence in situ hybridization (FISH) for the male rhesus monkey. We have recently generated a probe for FISH with high specificity to the short arm of the rhesus monkey Y chromosome. In this study, we further describe a method that keeps the integrity of tissue-specific antigenic structures for immunofluorescence staining subsequent to FISH on paraffin-embedded rhesus monkey tissues. We have examined this… CONTINUE READING
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